CytoD enabled osteogenic differentiation independently from the ECM, while it was rather blocked via Y-27632 treatment on FIB the general highest extent of differentiation occurred. Both inhibitors did not affect hASC proliferation. Except for the COL surface, both inhibitors increased the extent of osterix, while the Runx2 pathway was more sensitive to the culture condition. FIB significantly enhanced cell elongation even in presence of actin polymerization moreВ В» blockers cytochalasin D (CytoD) and ROCK inhibitor Y-27632, which generally caused more rounded cells. ECM guided differentiation was rather dependent on osterix than on Runx2 pathway. Proliferation and osteogenic markers like alkaline phosphatase activity and calcium mineralization were slightly increased by the ECM components laminin (LA), collagen I (COL), and fibronectin (FIB) with control medium no differentiation occurred. This interplay on osteogenic guidance of human adipose-derived stem cells (hASCs) was focus of this study. The correlation between extracellular matrix (ECM) components, cell shape, and stem cell guidance can shed light in understanding and mimicking the functionality of stem cell niches for various applications. This gives insight in tissue formation and is essential for biomedical applications.Cell behaviour follows a cell specific ligand-priority ranking.The ECM significantly guides all cell responses.We moreВ В» compare five different cell types, using the same culture conditions.We analyse the impact of ECM components on cell behaviour in vitro.This knowledge is essential for optimization of tissue-biomaterial interfaces in all tissue engineering applications and gives insight into tissue-specific cell guidance. Fibroblasts responded best to fibronectin, chondrocytes best to collagen I, the other cell types best to laminin. A detailed in vitro comparison of fibroblasts, endothelial cells, osteoblasts, smooth muscle cells, and chondrocytes reveals significant differences in their cell responses to the ECM: cell behaviour follows a cell specific ligand priority ranking, which was independent of the cell type origin. In this paper, we investigate the role of different ECM components like laminin, fibronectin, and collagen type I with respect to the essential cell behaviour patterns: attachment dynamics such as adhesion kinetic and force, formation of focal adhesion complexes, morphology, proliferation, and intercellular communication. Due to the complexity of ECM-cell interactions-which depend on the interplay of inside-out and outside-in signaling cascades, cell and tissue specificity of ECM-guidance is poorly understood. In this context, each tissue consists of a unique ECM composition, which may be responsible for tissue-specific cell responses. Cell binding to the extracellular matrix (ECM) is essential for cell and tissue functions.

Ecm cell